Saturday, February 08, 2014

Vaccine Contamination & Autism

VACCINE CONTAMINATION & AUTISM
- courtesy Lowell Hubbs

Human Diploid cells: MRC5 proteins:(human diploid cells, aborted fetuses)

Derived from normal lung tissue of a 14-week-old male fetus by J. P. Jacobs in September 1966 (Nature 227: 168-170, 1970), the MRC-5 cell line was established in a growth medium consisting of Earle's Basal Medium in Earle's balanced salt solution supplemented with 10% calf (bovine) serum. The MRC-5 cell strain (like the WI-38 cell line) is susceptible to a wide range of human viruses. 
http://www.viromed.com/services/product/mrc5.htm 

Viral vaccines are often cultured on aborted fetal tissue. There are several types of fetal cells in which the virus is grown: MRC5, WI-38, WI-26 VA4, RA273, PER C and HEK 293. This has raised concerns, with reports of some individuals with religious objections to abortion refusing vaccination because they feel this makes them complicit with the original act of abortion.

Some deem it unethical to use vaccines from fetal cell lines for the following reasons: 
1) If abortion is immoral then profiting from it must also be immoral. 
2) The unborn child was unable to give consent for its body parts to be used, so it is therefore disrespectful to the dead. 
3) The argument that "the fetal tissues would just go to waste if they were not used" was not accepted at the Nuremberg trials of scientists who used body parts from concentration camp victims. This abuse of the child's body only compounds the injustice of the original abortion, even if the vaccine producers were in no way connected to the abortion. 
4) Use of this vaccine implies acceptance of the legality of abortion, and does nothing to discourage the use of fetal parts or cell lines in vaccine manufacture or other branches of medicine.

Vaccines that use aborted fetuses:
Acambis 1000 (smallpox) Acambis -- MRC5
Avaxim (hepatitis A) Pateur Merieux -- MRC5
Biavax (mumps, rubella) Merck -- RA273, WI-38
Ervevax (rubella) Smithkilne Beecham -- MRC5
Havarix (hepatitis A) Smithkilne Beecham -- MRC5
Imovax HDCV, DCO (rabies) Pasteur Merieux Connaught -- MRC5
Meruvax II (rubella) Merck -- RA273, WI-38
MMR II (Measles, mumps, rubella) Merck Sharp & Dohme -- WI-38
MR VAX (measles, rubella) Merck -- RA273, WI-38
Poliovax (polio, IPV) MRC5
Priorix (measles, mumps, rubella) Smithkline Beecham -- MRC5 
Proquad (mmr, chickenpox/varicella) Merck -- RA273, WI-38, MRC5
Twinrix (hepatitis A and B) GSK -- MRC5
VAQTA (hepatitis A) MSD -- MRC5
Varivax (chickenpox, varicella) MSD -- WI-38
Zostavax (shingles) Merck -- MRC5, WI-38 

http://www.dgwsoft.co.uk/homepages/vaccines/alternatives.htm
http://www.cogforlife.org/fetalvaccines.htm

Source:
http://www.novaccine.com/vaccine-ingredients/results.asp?p=1&s=2&sc=14&scientific=Y

Is Aborted Fetal DNA in Vaccines Linked to Autism?
http://www.lifesitenews.com/news/archive//ldn/2009/jul/09072106

Fetal Tissue in Vaccine Production May be Linked to Autism in Children Claims Campaign Group
http://www.lifesitenews.com/news/archive//ldn/2009/jul/09070611

Study Confirms Autism Boom - Correlates with Aborted Fetal DNA in Vaccines
http://www.lifesitenews.com/news/archive/ldn/2010/apr/10042306

Abortion-Tainted New Flu Vaccine From Vaxin Uses Aborted Fetal Cell Lines
http://www.lifesitenews.com/ldn/2005/feb/05020703.html

Autism Research

The scientific community now knows that children with regressive autism have hundreds of de novo and diverse gene mutations. That means that regressive autism is not genetic. It must be triggered by an external event that can create hundreds of different DNA breaks and mutations. That has been obvious to us at SCPI since we were founded.

Since 2008, SCPI has been ahead of the field, doing the cutting edge biology, molecular modeling, computational informatics, and ecology to figure out what is causing so many DNA breaks and mutations in our children, long before the 2011 and 2012 publications put the hundreds of de novo mutations together. That’s because we knew that a condition associated with hundreds of different genes could not possibly be genetic. We knew that DNA and retroviral contaminants are present in some childhood vaccines and that these types of contaminants are known to cause DNA breaks and mutations.

Have we created the perfect storm for DNA breaks, mutations, and regressive autism in our children? In 1979 we started injecting our children with vaccines that are contaminated with aborted fetal DNA fragments and a retrovirus, and autism began to rise. Then we added more jabs with aborted fetal vaccines and thimerosal, which can also cause DNA breaks, to vaccines in 1988, and autism rose more. Then in 1995, we added much more aborted fetal DNA contaminants to the chickenpox vaccine, and autism really rose. And now we have children born to older dads who have sperm with very breakable DNA. Aborted fetal contaminated vaccines plus thimerosal plus older dads result in more DNA breaks, thus more de novo mutations, in our children.

Drugs and vaccines are too large to produce in a test tube, and therefore, they must be manufactured using cell lines. The final products contain contaminants from the cell line used to manufacture the drug or vaccine. When animal cell lines are utilized, these contaminants are recognized by our immune systems as ‘foreign’ and are eliminated from our bodies. However, when primitive human cell lines (such as an aborted fetal cell line) are used, these contaminants have the potential to trigger autoimmune diseases or genomic instability. When we use aborted fetal produced vaccines or cosmetics, we are also injecting or transferring DNA and viruses from the aborted fetus used to create the cell line into our own bodies.

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The contaminants found in vaccines and drugs that are manufactured using aborted fetal cell lines present the perfect storm of contaminants to cause genomic instability. Some childhood vaccines contain very high levels of short human fetal DNA fragments, which gene therapy studies have taught us are the perfect size to insert into our genes. Some childhood vaccines also contain a retroviral contaminant called HERVK, which is in the same family of retroviruses as the one that caused cancer in 4 of 9 boys.

Read more:
http://soundchoice.org/research/

DNA Fragments Research

•Vaqta Hepatitis A Vaccine is contaminated with MRC5 aborted fetal DNA and proteins (see page 1)
•MMR II is manufactured in WI 38 aborted fetal cell line
•Hepatitis A vaccine contaminated with MRC5 aborted fetal debris (see page 8)
•Chickenpox Contaminated with Human Fetal DNA (see page 3)
•Chickenpox and MMR II vaccines are contaminated with a retrovirus

http://soundchoice.org/research/dna-fragments-research/

Autism

The dangers of using aborted fetal cell lines for vaccine manufacture have been debated by the FDA for over 50 years, and yet they have not done sufficient safety studies. The active component of a vaccine is a virus. Viruses are too large to manufacture in test tubes. Therefore, vaccine manufacturers exploit the natural method of producing virus– they inoculate cells and the cells produce the virus for them. Each vial of vaccine contains contaminants from the cells used to make the virus. When we use animal cells to make viruses, the residual material is not human and so we mount an immune response to it and eliminate it. However, in the case of vaccines produced using aborted human fetal cell lines, we have the dangers of triggering an autoimmune response and insertion of the contaminating DNA to disrupt the child’s own genes.

In the US, autism has spiked up in 3 distinct years, called changepoints. The first changepoint occurred in 1981, the second in 19881, and the third in 1996. These spikes coincide with the introduction of vaccines that are produced in aborted fetal cells. In 1979, aborted fetal cell produced MMR II was approved in the US. Compliance campaigns brought MMR II use up from as low as 49% for children born before 1987 to over 82% for children born in 1989 and later. A second dose of MMR II was also introduced to the vaccination schedule for children born in 1988 and later. The third changepoint corresponds to the approval of aborted fetal cell produced Varivax (chickenpox) in 1995 (See figure below).

Read more: http://soundchoice.org/autism/

Did you know that some vaccines are made using aborted fetal cells?

Read the June 2013 Newsletter

1) Did you know that some vaccines are made using aborted fetal cells?

On June 24, we sent a survey to 3,738 people on our email list asking this question. Of the 1,138 people who have taken the survey so far, 23% (298) did not know that some childhood vaccines, as well as the shingles vaccine and a rabies vaccines, are manufactured using aborted fetal cell lines. Once people know how vaccines are manufactured, 94% of them do not feel comfortable using vaccines made in aborted fetal cell lines. The information is difficult to obtain because many pediatricians and other doctors do not know that the vaccines are manufactured using aborted fetal cells. This survey shows that we must do a better job of keeping people informed!

2) Did you know that those vaccines are contaminated with DNA, cellular debris, and in some cases, a retrovirus from the aborted fetus?

Surprisingly, only 50% of respondents said they were aware that some vaccines are contaminated with aborted fetal DNA and cellular debris. This response has let us know that we must do a better job explaining to people where the contaminants come from!

3) Our newsletters can keep you informed and up-to-date. Please read the whole newsletter so you don’t miss anything that you might want to know.

http://soundchoice.org/2013/07/01/did-you-know-that-some-vaccines-are-made-using-aborted-fetal-cells/

Aborted Fetal Vaccines
http://www.cogforlife.org/vaccineinfo.htm

Vaccines, Abortion, & Fetal Tissue 
http://media.rtl.org/pdf/VaccinesPrint.pdf

Vaccines using aborted fetal cell lines 
http://www.cogforlife.org/vaccineListOrigFormat.pdf

This study is as well another big strike against several of the currently made vaccines that are made by used of aborted fetal cells. Although the study article doesn't makes reference per say to human diploid cells, it rather uses terminology refering to human DNA residuals. That to me would be the same thing, or derived from the same thing. Human diploid cells of course can only come from and typically one thing, aborted fetal tissue.

The MMR vaccine is made by use of aborted fetal tissue; more specifically, MRC-5 and WI-38. For a list of other vaccines that are as well made with and by the use of human diploid tissue, a list of those vaccines is in the link below. 

Computational Detection of Homologous Recombination Hotspots in X-Chromosome Autism Associated Genes - Abstract

Friday, May 21, 2010
Franklin Hall B Level 4 (Philadelphia Marriott Downtown)
A. Ard , University of Portland, Portland, OR
M. LaMadrid , Sound Choice Pharmaceutical Institute, Seattle, WA
S. Bwabye , Sound Choice Pharmaceutical Institute, Seattle, WA
K. Koyama , Sound Choice Pharmaceutical Institute, Seattle, WA
T. Deisher , Sound Choice Pharmaceutical Institute, Seattle, WA

Background: Autism prevalence rates in the US and UK began increasing temporally close to the time that the MMR vaccine was switched from the type manufactured using animal cells to a type manufactured using human cells. A yet unstudied possible environmental cause for autism is residual human DNA contaminant in the vaccines produced using human cells. Debate about the dangers of residual human DNA in vaccines has been going on for 50 years, mainly related to cancer initiation. Clinical gene therapy (1996) of male infants has induced childhood leukemia due to improper DNA insertion. To minimize the possibility of oncogenic DNA integrating into a vaccine recipient’s genome, the WHO and FDA have recommended DNAse treatments to reduce the size of contaminant DNA fragments to less than genic lengths, ~200-1000bp. 

These recommendations were developed before the sequencing of the human genome. DNA cellular diffusion studies have shown that short DNA fragments (<250bp) have higher probability of entering the nucleus than longer lengths. Gene therapy studies have also demonstrated that naked DNA, injected intramuscularly, can remain intact and be transported to the brain via retrograde axonal transport in motor neurons. Once inside the nucleus, exogenous DNA can integrate via homologous recombination, potentially in genomic regions called ‘recombination hotspots’. Intra-species DNA integration, e.g., by homologous recombination, occurs with a probability a billion times greater than inter-species homologous recombination. Integration of short human DNA fragments has the potential to contribute to various human diseases, including autism.

Objectives: To verify the lengths of residual human DNA in vaccines and to determine if recombination hotspots occur near or in X-chromosome genes known to be associated with autism.

Methods: Human DNA from inactivated vaccines was isolated and characterized using standard procedures. A list of recombination hotspots, computationally derived by using Hapmap Phase II data, was downloaded. A list of 238 genes associated with autism (AAGs) was downloaded from the ACGMAP website. Gene coordinates, including transcription start and end sites, were downloaded from the UCSC Human Genome website. Software was written to automate the location of overlaps between autism associated genes and recombination hotspots.

Results: The average human DNA fragment length in rubella vaccine was 220 base pairs. Out of 1145 hotspots in the X-chromosome, 25 hotspots are located in 5 of 15 X-chromosome AAGs, between the transcription start and end sites. These genes are NLGN3 and NLGN4X (neuroligins involved in synapse formation), AFF2 and IL1RAPL1 (involved in X-linked mental retardation), and GRPR (gastrin releasing peptide receptor).

Conclusions: Autism-associated genes in the X-chromosome contain multiple regions where potential insertion of short, non-host homologous DNA can occur. With new knowledge due to the human genome project, particularly in regards to SNPs and epigenetics, further work must be done to understand the implications of integrated residual human DNA to the etiology of autism.

Computational Detection of Homologous Recombination Hotspots in X
http://download.pdf-world.net/Computational-Detection-of-Homologous-Recombination-Hotspots-in-X-download-w4554.html 

Excerpt from the abstract: 
http://www.cogforlife.org/scpiautisminsarabstract.htm 

June 3, 2010
SCPI Study on Aborted Fetal DNA in Vaccines Presented at International Meeting for Autism Research
http://www.cogforlife.org/scpiautismstudypress.htm

For Scientific Data: Homologous Recombination Study 
http://www.cogforlife.org/SCPIIMFARHR.pdf 

There is also shown in the study itself, what they call a Changepoint analysis for US(DOE) and CA(DDS) Autistic Disorder, Fig. 1. You can see the what appear to be direct increases in ASD after the use of vaccines containing human DNA residuals.

The study is said to be only a hypothesis, but none the less comes up with some very significant findings, not only in relation to autism, but as well in what would be clear resulting in potential adverse effects on health, irregardless of if actual ASD has resulted. 

Is There a Link Between Aborted Fetal DNA in Vaccines and Autism?
http://theaquilareport.com/is-there-a-link-between-aborted-fetal-dna-in-vaccines-and-autism/

Do you see how close it comes in use of aborted fetal cells in the use of vaccines, to changing the makeup of human genetics? This is man replacing the creator. Literally so, and thinking that man can improve on and make what was created, better. You can see how entirely misguided and oblivious to what potentially can happen, this is. Triggers for autism? They clearly do not have a clue, nor care to at pharma, FDA, and nor the CDC.

Using Homologous Recombination to Manipulate the Genome of Human Somatic Cells
http://download.pdf-world.net/Using-Homologous-Recombination-to-Manipulate-the-Genome-of-Human-download-w4540.html

Non-homologous DNA end joining - Acta Biochimica Polonica
http://download.pdf-world.net/Non-homologous-DNA-end-joining---Acta-Biochimica-Polonica-download-w4566.html

Mechanism of Eukaryotic Homologous Recombination
http://download.pdf-world.net/Mechanism-of-Eukaryotic-Homologous-Recombination-download-w4524.html

Genomic disorders: A window into human gene and genome evolution

Claudia M. B. Carvalhoa, Feng Zhanga, and James R. Lupskia,b,c,1
Author Affiliations
Edited by Diddahally R. Govindaraju, Boston University School of Medicine, Boston, MA, and accepted by the Editorial Board November 5, 2009 (received for review July 22, 2009)

Abstract
Gene duplications alter the genetic constitution of organisms and can be a driving force of molecular evolution in humans and the great apes. In this context, the study of genomic disorders has uncovered the essential role played by the genomic architecture, especially low copy repeats (LCRs) or segmental duplications (SDs). In fact, regardless of the mechanism, LCRs can mediate or stimulate rearrangements, inciting genomic instability and generating dynamic and unstable regions prone to rapid molecular evolution. In humans, copy-number variation (CNV) has been implicated in common traits such as neuropathy, hypertension, color blindness, infertility, and behavioral traits including autism and schizophrenia, as well as disease susceptibility to HIV, lupus nephritis, and psoriasis among many other clinical phenotypes. The same mechanisms implicated in the origin of genomic disorders may also play a role in the emergence of segmental duplications and the evolution of new genes by means of genomic and gene duplication and triplication, exon shuffling, exon accretion, and fusion/fission events.

http://www.pnas.org/content/107/suppl_1/1765.full

Vaccine Production With - Human Diploid Cells (aborted fetal cell tissue)
http://www.vacfacts.info/vaccine-production-with---human-diploid-cells-aborted-fetal-cell---tissue.html

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Here is a little more information; if you are unaware of this. An informational expose and a list of vaccines that have been made with human diploid cells, (shocking).

Children of God for Life: 
www.cogforlife.org

U.S. Vaccines Derived From Abortion
http://www.christianpatriot.com/vaccines.htm

USA AND CANADA - ABORTED FETAL CELL LINE PRODUCTS AND ETHICAL ALTERNATIVES, (not available for all vaccines)
http://www.cogforlife.org/vaccineListOrigFormat.pdf

MRC-5 (Lung, diploid, human)

Derived from normal lung tissue of a 14-week-old male fetus by J. P. Jacobs in September 1966 (Nature 227: 168-170, 1970), the MRC-5 cell line was established in a growth medium consisting of Earle's Basal Medium in Earle's balanced salt solution supplemented with 10% calf serum. Following initial cultivation, subcultures were prepared twice weekly at a 1:2 ratio. When the cells reached approximately the 7th population doubling, the majority of the cultures were harvested to prepare a frozen cell stock. Subsequent observations revealed that the MRC-5 cells are capable of attaining 42-46 population doublings before onset of the decline in proliferation usually experienced with human fibroblast lines. The MRC-5 cell strain (like the WI-38 cell). 

Culture Medium
Minimum essential medium (Eagle), with 10% heat inactivated fetal bovine serum. 

Growth Characteristics
Cells seeded at a concentration of 4x104 cells/cm2 in the above culture medium will be 100% confluent in 7 days. 

Plating Efficiency Less than 1%. Morphology Fibroblast-like. Karyology

Chromosome Frequency Distribution is 46 Cells: 2n = 46. 

Species: Confirmed as human by cytotoxic-antibody dye exclusion test.

Common Utilization
Supports the growth of a broad range of viruses, including Adenoviruses; Coxsackie A; Cytomegalovirus; Echovirus; Herpes simplex Virus; Poliovirus; Rhinovirus; Respiratory Syncytial Virus; and Varicella Zoster Virus. Also used for in Culture Medium

Source:
http://www.google.com/url?sa=t&rct=j&q=&esrc=s&frm=1&source=web&cd=2&cad=rja&ved=0CCsQFjAB&url=http%3A%2F%2Fsoundchoice.org%2Fwp-content%2Fuploads%2F2013%2F01%2FAppendix-J-MRC-5-derivation-and-NATURE-abstract.pdf&ei=vNL1Uq-5NInkyAHI5IHAAw&usg=AFQjCNEJLkdXjNVxcZ8edllcoqxs--i4RA&bvm=bv.60983673,d.aWc

Look healthy, in regard to injecting a vaccine made from these cells? Not at all.

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Vaccine contamination is an ongoing unresolved problem, and both the FDA and the CDC clearly know this.

SaneVax Announces Medical Surprise: Gardasil® HPV DNA Discovered in Post-Mortem Blood and Spleen Tissue
http://www.businesswire.com/news/home/20120808006480/en#.UvWdWn7Tncs

Dr. Lee is known for using the nested PCR/DNA sequencing technology for reliable detection and genotyping of HPV in clinical specimens. He is the author of the chapter, “Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papillomavirus from Clinical Specimens” in a Methods in Molecular Biology volume published by Humana Press in July 2012. 

Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma 

Virus from Clinical Specimens

Abstract

Accurate genotyping of a human papilloma virus (HPV) isolated from clinical specimens depends on molecular identification of the unique and exclusive nucleotide base sequence in the hypervariable region of a highly conserved segment of the HPV L1 gene. Among other options, a heminested (nested) polymerase chain reaction (PCR) technology using two consecutive PCR replications of the target DNA in tandem with three consensus general primers may be used to detect a minute quantity of HPV DNA in crude proteinase K digestate of cervicovaginal cells, and to prepare the template for genotyping by automated direct DNA sequencing. A short target sequence of 40–60 bases excised from the computer-generated electropherogram is sufficient for BLAST determination of all clinically relevant HPV genotypes, based on the database stored in the GenBank. This chapter discusses the principle and the essential technical elements in performing nested PCR DNA amplification for the detection of HPV from clinical specimens and short target sequence genotyping for HPV, using standard molecular biology laboratory equipment and commercially available reagents.

http://link.springer.com/protocol/10.1007%2F978-1-61779-937-2_5

Gardasil Contamination Article Archives
http://sanevax.org/tag/gardasil-contamination/

Unveiling the Culprit – Is Foreign DNA Contamination the Autistic Villain behind Biologic Vaccine Injuries?
http://holyhormones.com/womens-health/cancer-womens-health/cervical-cancer/unveiling-the-culprit-is-foreign-dna-contamination-the-autistic-villain-behind-biologic-vaccine-injuries/

Autism Epidemic, Is Foreign DNA in MMR II Vaccine Responsible? CBCD Suggests CDC Study Microcompetition Theory 

The Center for the Biology of Chronic Disease (CBCD) believes that the cause of the epidemic is the foreign DNA in the MMR II vaccine. 

http://www.prweb.com/releases/2012/4/prweb9359508.htm

Vaccine Contamination 
http://www.vacfacts.info/vaccine-contamination.html

I Am Against Gardasil, Dr. Doris Rapp
http://www.drrapp.com/2013/07/08/i-am-against-gardasil/

The Ugly Truth About Gardasil
http://www.drrapp.com/2011/11/02/the-ugly-truth-about-gardasil/

Brit Scientists Show HPV Vaccine Is Not Justified Anywhere
http://gaia-health.com/gaia-blog/2012-06-24/brit-scientists-show-hpv-vaccine-is-not-justified-anywhere/

Study: Evidence that Acetaminophen, Especially in Conjunction with Vaccines, is a Major Cause of Autism and Asthma
http://healthimpactnews.com/2013/study-evidence-that-acetaminophen-especially-in-conjunction-with-vaccines-is-a-major-cause-of-autism-and-asthma/

Empirical Data Confirm Autism Symptoms Related to Aluminum and Acetaminophen Exposure
http://people.csail.mit.edu/seneff/Entropy/entropy-14-02227.pdf

The Vaccine Damage Science
http://www.vacfacts.info/the-vaccine-damage---science.html

The Unbiased Vaccine Science and Data
http://www.vacfacts.info/the-unbiased-vaccine-science-and-data.html

Aluminum Adjuvants - Lack of Safety Data - Lack of Aluminum Adjuvant Safety Studies
http://www.vacfacts.info/aluminum-vaccine-adjuvants.html

Vaccine aluminum adjuvant causation of neuroglial activation and neuroinflammation in the brain of patients with autism.
http://www.vacfacts.info/vaccine-aluminum-adjuvant-causation-of-neuroglial-activation-and-neuroinflammation-in-the-brain-of-patients-with-autism.html

Vaccination toxicity: The Zeta phase of MASS and “blood sludging”
http://www.vacfacts.info/vaccination-toxicity-the-zeta-phase-of-mass-and-ldquoblood-sludgingrdquo.html

Vaccine Caused Ischemia/Hypoxia 
http://www.vacfacts.info/vaccine-caused-ischemiahypoxia.html

REDACTED VACCINE DEATH FORENSICS REPORT
Baby M's Parents,
http://www.vacfacts.info/baby-ms-parents-vaccine-death-forensics-report.html

Want A Strong Argument Against Vaccines, (more on vaccine contamination)
http://www.vacfacts.info/want-a-strong-argument-against-vaccines.html

If you can still tell me that none of the crude in vaccines is causing any problem nor autism, and that they remain safe and effective; all 49 doses of 14 vaccines, and 69 doses of 16 vaccines by the age 18; on the US CDC vaccine schedule; then you are not paying attention; and you are refusing to look at the evidence.

Modern Medicine and Vaccines - In A Nut Shell (an explanation of the existing battle against truth information; David, verses the misinformation and lies of the allopathic pharma, medical Goliath)
http://www.vacfacts.info/modern-medicine-and-vaccines---in-a-nut-shell.html



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