Friday, December 29, 2017

Fetal Cell Lines in Vaccines & Autism

 2015 Spring;30(1):47-70.

Epidemiologic and Molecular Relationship Between Vaccine Manufacture and Autism Spectrum Disorder Prevalence.

Abstract

OBJECTIVES:

To assess the public health consequences of fetal cell line manufactured vaccines that contain residual human fetal DNA fragments utilizing laboratory and ecological approaches including statistics, molecular biology and genomics.

METHOD:

MMR coverage and autism disorder or autism spectrum disorder prevalence data for Norway, Sweden and the UK were obtained from public and government websites as well as peer reviewed published articles. Biologically, the size and quantity of the contaminating fetal DNA in Meruvax II and Havrix as well as the propensity of various cell lines for cellular and nuclear uptake of primitive human DNA fragments were measured and quantified using gel electrophoresis, fluorescence microscopy and fluorometry. Lastly, genomic analysis identified the specific sites where fetal DNA fragment integration into a child's genome is most likely to occur.

RESULTS:

The average MMR coverage for the three countries fell below 90% after Dr. Wakefield's infamous 1998 publication but started to recover slowly after 2001 until reaching over 90% coverage again by 2004. During the same time period, the average autism spectrum disorder prevalence in the United Kingdom, Norway and Sweden dropped substantially after birth year 1998 and gradually increased again after birth year 2000. Average single stranded DNA and double stranded DNA in Meruvax II were 142.05 ng/vial and 35.00 ng/vial, respectively, and 276.00 ng/vial and 35.74 ng/vial in Havrix respectively. The size of the fetal DNA fragments in Meruvax II was approximately 215 base pairs. There was spontaneous cellular and nuclear DNA uptake in HFF1 and NCCIT cells. Genes that have been linked to autism (autism associated genes; AAGs) have a more concentrated susceptibility for insults to genomic stability in comparison to the group of all genes contained within the human genome. Of the X chromosome AAGs, 15 of 19 have double strand break motifs less than 100 kilobases away from the center of a meiotic recombination hotspot located within an exon.

CONCLUSION:

Vaccines manufactured in human fetal cell lines contain unacceptably high levels of fetal DNA fragment contaminants. The human genome naturally contains regions that are susceptible to double strand break formation and DNA insertional mutagenesis. The "Wakefield Scare" created a natural experiment that may demonstrate a causal relationship between fetal cell-line manufactured vaccines and ASD prevalence.

The concern being addressed (after the documentation of human DNA, at least in fragmented form) is whether or not such “vaccine DNA” could be incorporated into the living “human DNA” of the host recipient; however, that is only one rather minor concern with the injection of human DNA from one person’s cells into the body of another living human being. From an immunology and inflammology standpoint, the concern is the likelihood that exogenous human DNA would trigger inflammatory responses after being perceived by the immune system and received by receptors for DAMPs (damage associated molecular patterns). Whether this amount of inflammation triggered from human DNA in vaccines is great or small, frequent or uncommon is unknown due to the lack of adequate vaccine testing; however, one would expect it to be of greater consequence in “genetically susceptible” persons and also when co-administered in various mixtures with other vaccines and with their pro-inflammatory ingredients such as aluminum (which exacerbates the release of human DNA[1]), mercury (known to promote immune sensitization) and allergenic antibiotics common in vaccines, including streptomycin[2], polymyxin B[3], neomycin[4], gentamicin and kanamycin[5].
[1] "Although DNA DAMPs are closely associated with the development of autoimmune disease, DNA DAMPs also contribute to the activation of acquired immune responses following vaccination with alum adjuvant. Previous studies have shown that genomic DNA from dying cells induces the maturation of antigen-presenting cells as well as antigen-specific antibody and cytotoxic T cell responses. This suggests that self-DNA DAMPs can activate innate immune responses that induce acquired immunoresponses. Recently, Marichal et al. demonstrated that the adjuvanticity of alum was dependent on self-DNA released from cells at the alum inoculation site (Marichal et al., 2011). NLRP3 appears to be a key sensor in the induction of alum-mediated innate immunity, although its function is only partially dependent upon alum adjuvanticity. " Jounai et al. Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Front Cell Infect Microbiol. 2013 Jan 8;2:168 [2] Romano et al. Anaphylaxis to streptomycin. Allergy. 2002 Nov;57(11):1087-8 [3] Henao MP, Ghaffari G. Anaphylaxis to polymyxin B-trimethoprim eye drops. Ann Allergy Asthma Immunol. 2016 Apr;116(4):372 [4] Goh CL. Anaphylaxis from topical neomycin and bacitracin. Australas J Dermatol. 1986 Dec;27(3):125-6 [5] Sánchez-Pérez et al. Allergic contact dermatitis from gentamicin in eyedrops, with cross-reactivity to kanamycin but not neomycin. Contact Dermatitis. 2001 Jan;44(1):54

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